The procedure described by Okayama and Berg (1982), as later modified by Gubler and Hoffman (1983), is currently the most widely used technique for cDNA cloning. In the first step, firststranded cDNA is synthesized using the classical ...
Author: Valdis Berzins
Publisher: Springer Science & Business Media
In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling.
Author: Svein Valla
Publisher: Humana Press
The collection includes not only many updated and improved classic techniques but also a powerful group of advanced methods that point to future progress among them nonisotopic DNA labeling silver staining and automatic sequencing.
Author: Adrian J. Harwood
Publisher: Humana PressInc
This third edition of A Practical Guide to Molecular Cloning provides complete updates to all of the protocols, and incorporates much new material to cover a broader range of biomolecular techniques.
Author: Bernard Perbal
A A brief introduction to the methods for novel gene cloning , 33 A broad spectrum PCR method for the detection of polyomaviruses and avoidance of contamination by cloning vectors , 33 A century of ( epithelial ) transport physiology ...
Author: Stephen D. Fairbanks
Publisher: Nova Publishers